Test: GRE Subject Test: Biochemistry, Cell, and Molecular Biology

Two students have cloned a bacterial DNA polymerase into a mammalian expression vector. The students then transfected the vector into human tissue culture cell lines to determine if the DNA polymerase retains its native function in human cells.

1.

What technique is most suitable for the students to use to determine if the DNA polymerase is expressed in human cells?

Restriction digestion

Electrophoretic mobility shift assay (EMSA) 

Western blot

Polymerase chain reaction (PCR)

Electroporation

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