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Test: GRE Subject Test: Biochemistry, Cell, and Molecular Biology
1. | When performing whole-mount immunofluorescence of a specimen, background staining is often a concern with custom generated antibodies. Which of the following is not a way in which to increase specificity of the custom antibody? |
Generate the custom antibody against purified protein rather than a predictive peptide
Determine optimal blocking buffer components and concentrations
None of the other answers
Affinity purify the custom antisera
Determine optimal antibody dilution for the application by performing serial dilutions
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