Test: GRE Subject Test: Biology

A student researcher wants to clone the human hemoglobin gene (HBB) into an expression vector so that he can express HBB in mouse cells and observe the resulting phenotype. 

1.

Which sequence of techniques will allow the student researcher to successfully clone the HBB gene?

1. Use a restriction enzyme to digest the expression vector

2. Ligate HBB and the digested vector

3. Amplify HBB via PCR

1. Use a restriction enzyme to digest HBB

2. Amplify the expression vector via PCR 

3. Ligate the digested HBB and vector

1. Ligate HBB

2. Use a restriction enzyme to digest the expression vector 

3. Amplify HBB and the digested expression vector via PCR

1. Amplify HBB via PCR

2. Use a restriction enzyme to digest the expression vector

3. Ligate HBB and the digested vector

None of the other answers

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